Table of Contents

INTRODUCTION      
I. SAFETY IN THE LABORATORY

Unit Introduction

SAFETY PART 1: CREATING A SAFE WORKPLACE   
Fundamental Principles                                                                                     

Classroom Activity 1: Performing a Risk Assessment    
Classroom Activity 2: Exploring the CDC and OSHA Websites            
Classroom Activity 3: Responding to Emergencies     

SAFETY PART 2: WORKING SAFELY WITH CHEMICALSClassroom Activity 4: Understanding the Chemicals with which You Work
Fundamental Principles
Classroom Activity 5: Personal Protection
Laboratory Exercise 1: Tracking the Spread of Chemical Contamination   
Classroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure                                                  

SAFETY PART 3: WORKING SAFELY WITH BIOLOGICAL HAZARDS
Fundamental Principles  
Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production
Unit Discussion     
Safety Contract, student copy
Safety Contract, to turn in

II. DOCUMENTATION IN THE LABORATORY    
Unit Introduction        
Classroom Activity 7: Be an Auditor  
Classroom Activity 8: Writing and Following an SOP     
Laboratory Exercise 3: Keeping a Laboratory Notebook 
Unit Discussion

III. METROLOGY IN THE LABORATORY    
Unit Introduction 
Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant Figures 
Classroom Activity 9: Constructing a Simple Balance
Laboratory Exercise 5:Weight Measurements 1; Good Weighing Practices 
Laboratory Exercise 6: Weight Measurements 2; Performance Verification
Laboratory Exercise 7: Volume Measurements 1; Proper Use of Volume Measuring Devices
Laboratory Exercise 8: Volume Measurements 2; Performance Verification of a Micropipette
Laboratory Exercise 9: Measuring pH with Accuracy and Precision  
Unit Discussion

IV. SPECTROPHOTOMETRY
Unit Introduction  
Laboratory Exercise 10: Color and the Absorbance of Light     
Laboratory Exercise 11: Concentration, Absorbance, and Transmittance  
Laboratory Exercise 12: Preparing a Standard Curve with Food Coloring and Using it for Quantitation  
Classroom Activity 10:Beer’s Law and Calculating an Absorptivity Constant
Laboratory Exercise 13: Determination of the Absorptivity Constant for ONP   
Unit Discussion    

V. BIOLOGICAL SOLUTIONS
Unit Introduction   
Classroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering Chemicals  
Laboratory Exercise 14: Preparing Solutions with One Solute
Laboratory Exercise 15: Preparing Solutions to the Correct Concentration
Laboratory Exercise 16: Working with Buffers      
Laboratory Exercise 17: Preparing Breaking Buffer       
Laboratory Exercise 18: Preparing TE Buffer    
Laboratory Exercise 19: More Practice Making a Buffer 
Laboratory Exercise 20: Making a Quality Product in a Simulated Company
Unit Discussion  

VI. ASSAYS   
Unit Introduction  
Laboratory Exercise 21: Two Qualitative Assays      
Laboratory Exercise 22:UV Spectrophotometry of DNA; Quantitative Applications
Laboratory Exercise 23:UV Spectrophotometry of DNA, RNA, and Proteins; Qualitative Applications
Laboratory Exercise 24: Using Spectrophotometry for Quality Control; Niacin
Laboratory Exercise 25: A Quantitative Protein Assay; Learning the Assay
Laboratory Exercise 26: Exploring Assay Verification   
Laboratory Exercise 27: The Beta-Galactosidase Enzyme Assay 
Laboratory Exercise 28: Comparing the Specific Activity of Two Preparations of Beta-Galactosidase
Unit Discussion   

VII. SEPARATION METHODS  
Unit Introduction 
Classroom Activity 13: Separating Materials Using a Centrifuge, Planning
Laboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases      
Laboratory Exercise 30: Separation of Dyes Using Paper Chromatography
Laboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis
Laboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay
Laboratory Exercise 33: Optimizing Agarose Gel Electrophoresis
Laboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis
Unit Discussion

VIII. GROWING CELLS 
Unit Introduction
WORKING WITH BACTERIAL CELLS
Laboratory Exercise 35: Aseptic Technique on an Open Lab Bench 
Laboratory Exercise 36: Working with Bacteria on an Agar Substrate; Isolating Individual Colonies
Laboratory Exercise 37: Using a Compound Light Microscope
Laboratory Exercise 38: Gram Staining
Laboratory Exercise 39: Making Phosphate Buffered Saline
Laboratory Exercise 40: The Aerobic Spread Plate Method of Enumerating Colony Forming Units
Laboratory Exercise 41: Generation of an E. coli Growth Curve
WORKING WITH MAMMALIAN CELLS
Laboratory Exercise 42: Aseptic Technique in a Laminar Flow Hood
Laboratory Exercise 43: Making Ham’s F12 Medium from Dehydrated Powder
Laboratory Exercise 44: Examining, Photographing, and Feeding CHO (Chinese Hamster Ovary) Cells
Laboratory Exercise 45: Counting Cells Using a Hemacytometer
Laboratory Exercise 46: Subculturing CHO Cells
Laboratory Exercise 47: Preparing a Growth Curve For CHO Cells
Unit Discussion

APPENDICES
I Materials and Supplies that are Assumed to be Present in Your Laboratory
II Calculating Standard Deviation   
II Periodic Table of Elements