Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms

1. Culture Transfer Techniques

2. Techniques for Isolation of Pure Cultures

3. Cultural Characteristics of Microorganisms

Part 2: Microscopy

4. Microscopic Examination of Stained Cell Preparations

5. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

6. The Microscopic Measurement of Microorganisms

7. Darkfield Microscopy

Part 3: Bacterial Staining

8. Preparation of Bacterial Smears

9. Simple Staining

10. Negative Staining

11. Gram Stain

12. Acid-Fast Stain (Ziehl-Neelsen Method)

13. Differential Staining for Visualization of Bacterial Cell Structures

Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations

14. Nutritional Requirements: Media for the Routine Cultivation of Bacteria

15. Use of Differential and Selective Media

16. Physical Factors: Temperature

17. Physical Factors: pH of the Extracellular Environment

18. Physical Factors: Atmospheric Oxygen Requirements

19. Techniques for the Cultivation of Anaerobic Microorganisms

20. Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells

21. The Bacterial Growth Curve

Part 5: Biochemical Activities of Microorganisms

22. Extracellular Enzymatic Activities of Microorganisms

23. Carbohydrate Fermentation

24. Triple Sugar-Iron Agar Test

25. IMViC Test

26. Hydrogen Sulfide Test

27. Urease Test

28. Litmus Milk Reactions

29. Nitrate Reduction Test

30. Catalase Test

31. Oxidase Test

32. Utilization of Amino Acids

33. Genus Identification of Unknown Bacterial Cultures

Part 6: The Protozoa

34. Free-Living Protozoa

35. Parasitic Protozoa

Part 7: The Fungi

36. Cultivation and Morphology of Molds

37. Yeast Morphology, Cultural Characteristics, and Reproduction

38. Identification of Unknown Fungi

Part 8: The Viruses

39. Cultivation and Enumeration of Bacteriophages

40. Isolation of Coliphages from Raw Sewage

Part 9: Physical and Chemical Agents for the Control of Microbial Growth

41. Physical Agents of Control: Moist Heat

42. Physical Agents of Control: Environmental Osmotic Pressure

43. Physical Agents of Control: Electromagnetic Radiation

44. Chemical Agents of Control: Chemotherapeutic Agents

45. Determination of Penicillin Activity in the Presence and Absence of Penicillinase

46. Chemical Agents of Control: Disinfectants and Antiseptics

Part 10: Microbiology of Food

47. Microbiological Analysis of Food Products: Bacterial Count

48. Wine Production

49. Sauerkraut Production

Part 11: Microbiology of Water

50. Standard Qualitative Analysis of Water

51. Quantitative Analysis of Water: Membrane Filter Method

Part 12: Microbiology of Soil

52. Nitrogen Cycle

53. Microbial Populations in Soil: Enumeration

54. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates

55. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

Part 13: Bacterial Genetics

56. Enzyme Induction

57. Bacterial Conjugation

58. Isolation of a Streptomycin-Resistant Mutant

59. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity

Part 14: Biotechnology

60. Bacterial Transformation

61. Isolation of Bacterial Plasmids

62. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

Part 15: Medical Microbiology

63. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries

64. Normal Microbial Flora of the Throat and Skin

65. Identification of Human Staphylococcal Pathogens

66. Identification of Human Streptococcal Pathogens

67. Identification of Streptococcus pneumoniae

68. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems

69. Isolation and Presumptive Identification of Campylobacter

70. Microbiological Analysis of Urine Specimens

71. Microbiological Analysis of Blood Specimens

72. Species Identification of Unknown Bacterial Cultures

Part 16: Immunology

73. Precipitin Reaction: The Ring Test

74. Precipitin Reaction: Immunodiffusion

75. Agglutination Reaction: The Febrile Antibody

76. Immunofluorescence

77. Enzyme-Linked Immunoabsorbent Assay

78. Agglutination Reaction: Mono-Test for Infectious Mononucleosis

79. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures